Fig. 3. Leukocyte chemoattractant levels and accumulation are decreased with AT treatment. Peritoneal lavage (PL) and blood were collected for cellular and cytokine analysis, respectively. A) Neutrophil accumulation and B) serum KC levels were determined for septic control and AT-treated mice three hours after CLP. *  p<0.05 vs CLP+NS; n = 8 mice/group. C) Neutrophil chemotaxis toward KC was determined in an in vitro transwell assay and analyzed by flow cytometry. * p<0.05 vs NS; n = 8 replicates in 2 independent experiments. D) Monocyte accumulation and E) serum MCP-1 levels were determined for septic control and AT-treated mice three hours after CLP. * p<0.05 vs CLP+NS; n = 8 mice/group.